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Drosera | Sarracenia | Dionaea | Nepenthes | Pinguicula | Utricularia | Darlingtonia | Basic Sterilization | Gibberellic Acid | How to TC

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Pinguicula

Difficulty:      Moderate
Usable Parts:      Seeds, leaves, flower stalks?

Sterilization:

Seeds:
10 min. bleach | 2 - 5 min. rinse or 1 - 10 min. rinse
or
Sterilize by soaking in 2% PPM 4 hrs with no rinse - I use this for all seeds and it works great all the time

Leaves
Alcohol dip | 20 min bleach | 2 - 5 min rinse or 1 - 15 min rinse
or
Alcohol dip | 10 min bleach | 2 - 5 min rinse | 1 hr 5% PPM | No rinse, transfer directly to media
or
See the forum for NaDCC instructions

Different leaf types will need treated differently.
Media:
4 cups water | 1/2 teaspoon MS w/Vitamins (50% MS) | 2 tablespoons sugar (25g) | 1 ml PPM | PH 5.6


Some leaves and most flower stalks can be used. I will try and get this section completed sometime this year. Tissue culture stalks in above media and sterilize per sundew flower stalk.




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since April 20, 2005



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